# Semi-synthetic DNA–protein conjugates: novel tools in analytics and nanobiotechnology

> This article reports on the syntheses, characterization and applications of semi-synthetic conjugates composed of nucleic acids, proteins and inorganic nanoparticles. For example, self-assembled oligomeric networks consisting of streptavidin and double-stranded DNA are applicable as reagents in immu...

## Metadata
- Authors: Christof M. Niemeyer
- Journal: Biochemical Society Transactions
- Published: 2004-02-01
- DOI: https://doi.org/10.1042/bst0320051
- Citations: 38
- Source: OpenAlex

## Technology Hub
- Hub: Semiconductor Technology
- Discipline: Engineering / Materials Science
- Hub URL: https://science-database.com/technology/semiconductor
- Hub llms.txt: https://science-database.com/technology/semiconductor/llms.txt

## Abstract
This article reports on the syntheses, characterization and applications of semi-synthetic conjugates composed of nucleic acids, proteins and inorganic nanoparticles. For example, self-assembled oligomeric networks consisting of streptavidin and double-stranded DNA are applicable as reagents in immunoassays, model systems for ion-switchable nanoparticle networks as well as nanometer-scaled 'soft material' standards for scanning probe microscopy. Covalent conjugates of single-stranded DNA and streptavidin are utilized as biomolecular adapters for the immobilization of biotinylated macromolecules at solid substrates via nucleic acid hybridization. This 'DNA-directed immobilization' allows for reversible and site-selective functionalization of solid substrates with metal and semiconductor nanoparticles or, vice versa, for the DNA-directed functionalization of gold nanoparticles with proteins, such as immunoglobulins and enzymes. This approach is applicable for the detection of chip-immobilized antigens. Moreover, covalent DNA-protein conjugates allow for their selective positioning along single-stranded nucleic acids, and thus for the construction of nanometre-scale assemblies composed of proteins and/or nanoclusters. Examples include the fabrication of functional biometallic nanostructures from gold nanoparticles and antibodies, applicable as diagnostic tools in bioanalytics.

## Links
- DOI: https://doi.org/10.1042/bst0320051
- OpenAlex: https://openalex.org/W2109465126
- JSON API: https://science-database.com/api/v1/technology/semiconductor

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Paper ID: oa-W2109465126 | Hub: semiconductor