# Off-target effects in CRISPR/Cas9 gene editing

> To date, many methods have been developed to nominate or detect the off-target sites of CRISPR/Cas9, which laid the basis for the successful upgrades of CRisPR/ Cas9 derivatives with enhanced precision, and about the current challenges in the management of off- target effects for future gene therapy.

## Metadata
- Authors: Congting Guo, Xiaoteng Ma, Fei Gao, Yuxuan Guo
- Journal: Frontiers in Bioengineering and Biotechnology
- Published: 2023
- DOI: https://doi.org/10.3389/fbioe.2023.1143157
- Citations: 402
- Source: Semantic Scholar
- Access: Open Access

## Technology Hub
- Hub: CRISPR Gene Editing
- Discipline: Biology / Genetics
- Hub URL: https://science-database.com/technology/crispr
- Hub llms.txt: https://science-database.com/technology/crispr/llms.txt

## Abstract
Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient, convenient and programmable, leading to promising translational studies and clinical trials for both genetic and non-genetic diseases. A major concern in the applications of the CRISPR/Cas9 system is about its off-target effects, namely the deposition of unexpected, unwanted, or even adverse alterations to the genome. To date, many methods have been developed to nominate or detect the off-target sites of CRISPR/Cas9, which laid the basis for the successful upgrades of CRISPR/Cas9 derivatives with enhanced precision. In this review, we summarize these technological advancements and discuss about the current challenges in the management of off-target effects for future gene therapy.

## Links
- DOI: https://doi.org/10.3389/fbioe.2023.1143157
- Semantic Scholar: https://www.semanticscholar.org/paper/ee3fc4548be78b7118b82051f44a3e6c4ce8ef78
- PDF: https://www.frontiersin.org/articles/10.3389/fbioe.2023.1143157/pdf
- JSON API: https://science-database.com/api/v1/technology/crispr

---
Generated by science-database.com — The Knowledge Interface
Paper ID: s2-ee3fc4548be78b7118b82051f44a3e6c4ce8ef78 | Hub: crispr