# Current applications and future perspective of CRISPR/Cas9 gene editing in cancer

> The basic principles ofCRISPR/Cas9 gene editing are explained and several new CRISPR-based gene editing modes are introduced, and the rapid progress of CRISpr screening in revealing tumorigenesis, metastasis, and drug resistance mechanisms are detailed.

## Metadata
- Authors: Si-wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-yong Huang, Jia-Bin Cai, Peng-fei Zhang, Yuexin Cui, A. Ke
- Journal: Molecular Cancer
- Published: 2022
- DOI: https://doi.org/10.1186/s12943-022-01518-8
- Citations: 358
- Source: Semantic Scholar
- Access: Open Access

## Technology Hub
- Hub: CRISPR Gene Editing
- Discipline: Biology / Genetics
- Hub URL: https://science-database.com/technology/crispr
- Hub llms.txt: https://science-database.com/technology/crispr/llms.txt

## Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This system inspires the development of a powerful genome engineering tool, the CRISPR/CRISPR-associated nuclease 9 (CRISPR/Cas9) genome editing system. Due to its high efficiency and precision, the CRISPR/Cas9 technique has been employed to explore the functions of cancer-related genes, establish tumor-bearing animal models and probe drug targets, vastly increasing our understanding of cancer genomics. Here, we review current status of CRISPR/Cas9 gene editing technology in oncological research. We first explain the basic principles of CRISPR/Cas9 gene editing and introduce several new CRISPR-based gene editing modes. We next detail the rapid progress of CRISPR screening in revealing tumorigenesis, metastasis, and drug resistance mechanisms. In addition, we introduce CRISPR/Cas9 system delivery vectors and finally demonstrate the potential of CRISPR/Cas9 engineering to enhance the effect of adoptive T cell therapy (ACT) and reduce adverse reactions.

## Links
- DOI: https://doi.org/10.1186/s12943-022-01518-8
- Semantic Scholar: https://www.semanticscholar.org/paper/cda0679e86ce8af508a4e5b83b587d4be183cd70
- PDF: https://molecular-cancer.biomedcentral.com/track/pdf/10.1186/s12943-022-01518-8
- JSON API: https://science-database.com/api/v1/technology/crispr

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Paper ID: s2-cda0679e86ce8af508a4e5b83b587d4be183cd70 | Hub: crispr