# Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing

> Gene editing using CRISPR/Cas9 may provide a new therapeutic path for eliminating HIV-1 DNA from CD4+ T-cells and potentially serve as a novel and effective platform toward curing AIDS.

## Metadata
- Authors: R. Kamiński, Yilan Chen, Tracy Fischer, E. Tedaldi, A. Napoli, Yonggang Zhang, J. Karn, Wenhui Hu, K. Khalili
- Journal: Scientific Reports
- Published: 2015
- DOI: https://doi.org/10.1038/srep22555
- Citations: 295
- Source: Semantic Scholar
- Access: Open Access

## Technology Hub
- Hub: CRISPR Gene Editing
- Discipline: Biology / Genetics
- Hub URL: https://science-database.com/technology/crispr
- Hub llms.txt: https://science-database.com/technology/crispr/llms.txt

## Abstract
We employed an RNA-guided CRISPR/Cas9 DNA editing system to precisely remove the entire HIV-1 genome spanning between 5′ and 3′ LTRs of integrated HIV-1 proviral DNA copies from latently infected human CD4+ T-cells. Comprehensive assessment of whole-genome sequencing of HIV-1 eradicated cells ruled out any off-target effects by our CRISPR/Cas9 technology that might compromise the integrity of the host genome and further showed no effect on several cell health indices including viability, cell cycle and apoptosis. Persistent co-expression of Cas9 and the specific targeting guide RNAs in HIV-1-eradicated T-cells protected them against new infection by HIV-1. Lentivirus-delivered CRISPR/Cas9 significantly diminished HIV-1 replication in infected primary CD4+ T-cell cultures and drastically reduced viral load in ex vivo culture of CD4+ T-cells obtained from HIV-1 infected patients. Thus, gene editing using CRISPR/Cas9 may provide a new therapeutic path for eliminating HIV-1 DNA from CD4+ T-cells and potentially serve as a novel and effective platform toward curing AIDS.

## Links
- DOI: https://doi.org/10.1038/srep22555
- Semantic Scholar: https://www.semanticscholar.org/paper/8b9b236b38ce529c71a4f1aae7f3f9fa95216b1f
- PDF: https://doi.org/10.1016/s2055-6640(20)31337-6
- JSON API: https://science-database.com/api/v1/technology/crispr

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Paper ID: s2-8b9b236b38ce529c71a4f1aae7f3f9fa95216b1f | Hub: crispr