# A boronic acid–rich dendrimer with robust and unprecedented efficiency for cytosolic protein delivery and CRISPR-Cas9 gene editing

> The rational design of a polymer with unprecedented and robust efficiency for cytosolic delivery of native proteins and ensures efficient intracellular delivery of Cas9 protein into various cell lines and showed high efficiency in CRISPR-Cas9 genome editing.

## Metadata
- Authors: Chongyi Liu, Tao Wan, Hui Wang, Song Zhang, Y. Ping, Yiyun Cheng
- Journal: Science Advances
- Published: 2019
- DOI: https://doi.org/10.1126/sciadv.aaw8922
- Citations: 293
- Source: Semantic Scholar
- Access: Open Access

## Technology Hub
- Hub: CRISPR Gene Editing
- Discipline: Biology / Genetics
- Hub URL: https://science-database.com/technology/crispr
- Hub llms.txt: https://science-database.com/technology/crispr/llms.txt

## Abstract
We report the rational design of a polymer with unprecedented and robust efficiency for cytosolic delivery of native proteins. Cytosolic protein delivery is of central importance for the development of protein-based biotechnologies and therapeutics; however, efficient intracellular delivery of native proteins remains a challenge. Here, we reported a boronic acid–rich dendrimer with unprecedented efficiency for cytosolic delivery of native proteins. The dendrimer could bind with both negatively and positively charged proteins and efficiently delivered 13 cargo proteins into the cytosol of living cells. All the delivered proteins kept their bioactivities after cytosolic delivery. The dendrimer ensures efficient intracellular delivery of Cas9 protein into various cell lines and showed high efficiency in CRISPR-Cas9 genome editing. The rationally designed boronic acid–rich dendrimer permits the development of an efficient platform with high generality for the delivery of native proteins.

## Links
- DOI: https://doi.org/10.1126/sciadv.aaw8922
- Semantic Scholar: https://www.semanticscholar.org/paper/334a60ae88d3926699991115a8ad4f632c302ed1
- PDF: https://advances.sciencemag.org/content/advances/5/6/eaaw8922.full.pdf
- JSON API: https://science-database.com/api/v1/technology/crispr

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Paper ID: s2-334a60ae88d3926699991115a8ad4f632c302ed1 | Hub: crispr